FIGURE 1:

Protein gel data. CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and stained  with Coomassie blue. The migration and molecular  weight of the protein standards are indicated. Uncharged  pAG-Tn5 monomer is 78.5 kDa, however once charged  with DNA, Tn5 dimerizes to a final complex weight of 191 kDa.

FIGURE 2:

Size distribution of released chromatin. CUT&Tag was performed as described above. Recovered DNA was directly PCR amplified to produce sequenceready libraries. Agilent TapeStation traces for libraries derived from negative control IgG (top) and H3K27me3 (bottom) antibodies are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size).

FIGURE 3:

CUT&Tag data. CUT&Tag was performed as described above. Representative sequencing tracks obtained using CUTANA pAG-Tn5 show a 229 kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced clear peaks with genomic distribution profiles consistent with the known biological functions of H3K4me3 and H3K27me3 as well as minimal background in the IgG negative control.

15-1017

CUTANA™ pAG-Tn5 for CUT&Tag

50 Reactions

15-1117

CUTANA™ pAG-Tn5 for CUT&Tag

250 Reactions