Description

Trusted TriLink quality, exceptional price. A key step in cellular mRNA processing is the addition of a 5’ cap structure, which is a 5'-5' triphosphate linkage between the 5' end of the RNA and a guanosine nucleotide. The cap is methylated enzymatically at the N-7 position of the guanosine to form mature mCAP. When preparing synthetic mRNA, the cap is often added prior to use in order to stabilize the mRNA and significantly enhance translation. Using a 4:1 mixture of a cap analog to GTP in transcription reactions will cap 80% of the resulting mRNAs. If mCAP (pre-methylated CAP) is used, only half of the asymmetrical mCAP will insert in the functional orientation. ARCA can only insert in the correct orientation, resulting in mRNAs that are translated twice as efficiently. TriLink is the least expensive source of high quality ARCA. We also offer CAP and mCAP for more traditional capping reactions.

Product Details

Catalog NoN-7003

Purity≥95% by AX-HPLC

Extinction Coefficient21,000 Lmol-1cm-1 at 254 nm

Molecular FormulaC22H32N10O18P3 (free acid)

Molecular Weight817.40 g/mole (free acid)

Salt FormNH4+

Concentration100 mM

BufferH2O

Recommended Storage-20°C or below

Other Name(s)Anti Reverse Cap Analog, 3′-O-Me-m7G(5')ppp(5')G

ApplicationCRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases

Backbone5'-5'-Triphosphate

Base Analog(s)Guanosine

Nucleotide CategoryCap analogs